Show simple item record

dc.contributor.authorViera Vicario, Alberto 
dc.contributor.authorParra Catalán, María Teresa 
dc.contributor.authorArévalo, Sara
dc.contributor.authorGarcía de la Vega, Carlos 
dc.contributor.authorSantos, Juan Luis
dc.contributor.authorPage Utrilla, Jesús 
dc.contributor.otherUAM. Departamento de Biologíaes_ES
dc.date.accessioned2022-10-24T10:11:20Z
dc.date.available2022-10-24T10:11:20Z
dc.date.issued2021-11-23
dc.identifier.citationGenes 12.12 (2021): 1844es_ES
dc.identifier.issn2073-4425es_ES
dc.identifier.urihttp://hdl.handle.net/10486/704712
dc.description.abstractRegulation of transcriptional activity during meiosis depends on the interrelated processes of recombination and synapsis. In eutherian mammal spermatocytes, transcription levels change during prophase-I, being low at the onset of meiosis but highly increased from pachytene up to the end of diplotene. However, X and Y chromosomes, which usually present unsynapsed regions throughout prophase-I in male meiosis, undergo a specific pattern of transcriptional inactivation. The interdependence of synapsis and transcription has mainly been studied in mammals, basically in mouse, but our knowledge in other unrelated phylogenetically species is more limited. To gain new insights on this issue, here we analyzed the relationship between synapsis and transcription in spermatocytes of the grasshopper Eyprepocnemis plorans. Autosomal chromosomes of this species achieve complete synapsis; however, the single X sex chromosome remains always unsynapsed and behaves as a univalent. We studied transcription in meiosis by immunolabeling with RNA polymerase II phosphorylated at serine 2 and found that whereas autosomes are active from leptotene up to diakinesis, the X chromosome is inactive throughout meiosis. This inactivation is accompanied by the accumulation of, at least, two repressive epigenetic modifications: H3 methylated at lysine 9 and H2AX phosphorylated at serine 139. Furthermore, we identified that X chromosome inactivation occurs in premeiotic spermatogonia. Overall, our results indicate: (i) transcription regulation in E. plorans spermatogenesis differs from the canonical pattern found in mammals and (ii) X chromosome inactivation is likely preceded by a process of heterochromatinization before the initiation of meiosises_ES
dc.format.extent17 pag.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relation.ispartofGeneses_ES
dc.rights© 2021 by the authorses_ES
dc.subject.otherAnimalses_ES
dc.subject.otherChromosome Pairinges_ES
dc.subject.otherEpigenesis, Genetices_ES
dc.subject.otherFemalees_ES
dc.subject.otherGene Silencinges_ES
dc.subject.otherGrasshopperses_ES
dc.subject.otherLysinees_ES
dc.subject.otherMalees_ES
dc.subject.otherMeiosises_ES
dc.subject.otherMeiotic Prophase Ies_ES
dc.subject.otherRNA Polymerase IIes_ES
dc.subject.otherSpermatocyteses_ES
dc.subject.otherSpermatogenesises_ES
dc.subject.otherX Chromosomees_ES
dc.subject.otherX Chromosome Inactivationes_ES
dc.subject.otherY Chromosomees_ES
dc.titleX chromosome inactivation during grasshopper spermatogenesises_ES
dc.typearticlees_ES
dc.subject.ecienciaBiología y Biomedicina / Biologíaes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/genes12121844es_ES
dc.identifier.doi10.3390/genes12121844es_ES
dc.identifier.publicationfirstpage1844-1es_ES
dc.identifier.publicationissue12es_ES
dc.identifier.publicationlastpage1844-17es_ES
dc.identifier.publicationvolume12es_ES
dc.relation.projectIDGobierno de España. BFU2009-10987es_ES
dc.relation.projectIDGobierno de España. CGL2014-53106-Pes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dc.rights.ccReconocimientoes_ES
dc.rights.accessRightsopenAccesses_ES
dc.facultadUAMFacultad de Cienciases_ES


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record