Interfacing neurons with nanostructured electrodes modulates synaptic circuit features
EntityUAM. Departamento de Física de la Materia Condensada
10.1002/adbi.202000117Advanced Biosystems 4.9 (2020): 2000117
Projectinfo:eu-repo/grantAgreement/EC/H2020/737116/EU//ByAxon; Gobierno de España. SEV-2016-0686; Comunidad de Madrid. S2018/NMT-4321/NanomagCOST-CM
SubjectsChemical Compositions; Electrical Stimulations; Intracellular Calcium; Microelectrode Array; Nano-Structured Electrodes; Nanostructured Substrates; Neuronal Differentiation; Physicochemical Features; Física
Rights© 2020 The Authors
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial 4.0 Internacional.
Understanding neural physiopathology requires advances in nanotechnology-based interfaces, engineered to monitor the functional state of mammalian nervous cells. Such interfaces typically contain nanometer-size features for stimulation and recording as in cell-non-invasive extracellular microelectrode arrays. In such devices, it turns crucial to understand specific interactions of neural cells with physicochemical features of electrodes, which could be designed to optimize performance. Herein, versatile flexible nanostructured electrodes covered by arrays of metallic nanowires are fabricated and used to investigate the role of chemical composition and nanotopography on rat brain cells in vitro. By using Au and Ni as exemplary materials, nanostructure and chemical composition are demonstrated to play major roles in the interaction of neural cells with electrodes. Nanostructured devices are interfaced to rat embryonic cortical cells and postnatal hippocampal neurons forming synaptic circuits. It is shown that Au-based electrodes behave similarly to controls. Contrarily, Ni-based nanostructured electrodes increase cell survival, boost neuronal differentiation, and reduce glial cells with respect to flat counterparts. Nonetheless, Au-based electrodes perform superiorly compared to Ni-based ones. Under electrical stimulation, Au-based nanostructured substrates evoke intracellular calcium dynamics compatible with neural networks activation. These studies highlight the opportunity for these electrodes to excite a silent neural network by direct neuronal membranes depolarization
Google Scholar:Domínguez-Bajo, Ana - Rodilla, Beatriz Loreto - Calaresu, Ivo - Arché-Núñez, Ana - González-Mayorga, Ankor - Scaini, Denis - Pérez, Lucas - Camarero de Diego, Julio - Miranda Soriano, Rodolfo - López-Dolado, Elisa - González, María Teresa - Ballerini, Laura - Serrano, María Concepción
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