Azure A embedded in carbon dots as NADH electrocatalyst: Development of a glutamate electrochemical biosensor
EntityUAM. Departamento de Química Analítica y Análisis Instrumental
10.1016/j.snb.2022.132761Sensors and Actuators: B. Chemical 374 (2023): 132761
Funded byThis work has been supported by the Spanish Ministerio de Ciencia e Innovacion (PID2020–116728RB-I00) and Comunidad Autonoma de Madrid (SI3/PJI/2021–00341, P2018/NMT-4349 TRANSNANOAVANSENS Program)
ProjectGobierno de España. PID2020–116728RB-I00
SubjectsAzure A; Carbon nanodot; Glutamate Biosensor; Glutamate dehydrogenase; NADH; Química
Rights© 2022 The Author(s)
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
Carbon nanodots modified with azure A (AA-CDs) have been synthesized and applied as redox mediator of bioelectrocatalytic reactions. A deep characterization of AA-CDs nanomaterial has been carried out, proving the covalent attachment of azure A molecules into the carbon dots nanostructure. Disposable screen-printed carbon electrodes (SPCE) have been modified with AA-CDs, through the action of chitosan polymer (Chit-AA-CDs/SPCE). The Chit-AA-CDs/SPCE electrocatalytic activity towards the oxidation of NADH has been proved, obtaining excellent results regarding the low oxidation potential achieved (−0.15 V vs. Ag) and low detection and quantification limits (LOD and LOQ) for NADH, 16 and 53 µM, respectively. The developed electrochemical platform has been applied for the construction of a glutamate biosensor by immobilizing L-glutamic dehydrogenase (GLDH/Chit-AA-CDs/SPCE). The morphology of GLDH/Chit-AA-CDs/SPCE platform was analysed by AFM at each different step of the electrode modification process. The resulting biosensing platform is capable of detect NADH enzymatically generated by GLDH in the presence of glutamate and NAD+. Good analytical parameters were obtained for glutamate analysis using GLDH/Chit-AA-CDs/SPCE, as LOD and LOQ of 3.3 and 11 µM, respectively. The biosensor has been successfully applied to the analysis of food and biological samples
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Google Scholar:Martínez Periñán, Emiliano - Domínguez Saldaña, Aitor - Villa Manso, Ana M. - Gutiérrez Sánchez, María Cristina - Revenga Parra, Mónica - Mateo Martí, Eva - Pariente Alonso, Félix - Lorenzo Abad, Encarnación
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