An optimized protocol for coupling oxygen consumption rates with β-oxidation in isolated mitochondria from mouse soleus
Entity
UAM. Departamento de Biología Molecular; UAM. Departamento de MedicinaPublisher
Cell PressDate
2021-09-17Citation
10.1016/j.xpro.2021.100735
Star Protocols 2.3 (2021): 100735
ISSN
2666-1667 (online)DOI
10.1016/j.xpro.2021.100735Project
Gobierno de España. PID2019-104241RB-I00Editor's Version
https://doi.org/10.1016/j.xpro.2021.100735Subjects
Cells, Cultured; Centrifugation; Mice; Mice, Inbred C57BL; Mitochondria, Muscle; Muscle, Skeletal; Oxidation-Reduction; Oxygen; Oxygen Consumption; Spectrophotometry; FísicaRights
© 2021 The Author(s)Abstract
Depending on metabolic requirements, skeletal muscle mitochondria integrate
O2 consumption and ATP production with lipid, glucose, or amino acid metabolism. Free fatty acids (FFAs) are the main source of energy during rest and
mild-intensity exercise. We present a detailed protocol for measuring FFAb-oxidation coupled with O2 respiration by a Clark-type electrode in isolated
mitochondria from mouse soleus oxidative muscle. We optimized the procedure,
including buffer composition, protease treatment, and quantifiable parameters
(P/O, Phosphate/Oxygen Ratio; OCR, Oxygen Consumption Rate; RCR,Respiration Control Rate; OSR, Oligomycin Sensitive Respiration).
For complete details on the use and execution of this protocol, please refer to
Sanchez-Gonzalez et al. (2020)
Files in this item
Google Scholar:Sánchez González, Cristina
-
Formentini, Laura
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