Effect of Gold Nanostars Plus Amikacin against Carbapenem-Resistant Klebsiella pneumoniae Biofilms
Fecha de edición2022-01-20
10.3390/biology11020162Biology 11.2 (2022) 1-17
Financiado porThis research was funded by the European Research Council through ERC-2015-AdG694160 (VERDI) grant. AM is funded by grants from Instituto de Salud Carlos III through the “Miguel Servet” program (CP15/00053)
ProyectoGobierno de España. CP15/00053; info:eu-repo/grantAgreement/EC/H2020/694160/EU//VERDI
Versión del editorhttps://doi.org/10.3390/biology11020162
MateriasAmikacin; Biofilm; Carbapenem-resistant K. pneumoniae; Gold nanostars; Medicina
Derechos© 2022 by the authors
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
(1) Background: Carbapenem-resistant Klesiella pneumoniae (CR-KP) infection rates depict an almost pre-antibiotic scenario since the pipeline for effective antibiotics against this pathogen has been almost entirely depleted. This study aims to evaluate the antibacterial effect of gold nanostars (GNS) alone or associated with some of the most widely used antibiotics for the treatment of CR-KP strains, i.e., meropenem or amikacin, on both planktonic and sessile forms. Additionally, we measured the effect of GNS on cell proliferation and biocompatibility in invertebrate in vivo models. (2) Materials and methods: GNS were made from gold seeds grown using a seeded-growth surfactant-free method assisted by silver ions and functionalized with mercapto-poly(ethylene glycol)amino by ligand exchange. The antimicrobial capacity, effect on cell proliferation, and biocom-patibility of the most effective combination was evaluated in a Galleria mellonella model. (3) Results: The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were 80 and 160 µM of GNS for all strains, respectively. The minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were >320 µM of GNS for both. A synergy was found between GNS and amikacin. Larvae administered GNS plus amikacin were found to tolerate the treatment well, which prevented infection. (4) Conclusions: GNS are a promising anti-CR-KP nanomaterial.
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Google Scholar:Aguilera-Correa, John Jairo - García-álvarez, Rafaela - Mediero Muñoz, Aránzazu - Esteban Moreno, Jaime - Vallet-Regí, María
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