Surface characterization of alkane viral anchoring films prepared by titanate-assisted organosilanization
EntityUAM. Departamento de Física Aplicada; UAM. Departamento de Física de la Materia Condensada; UAM. Departamento de Física de Materiales
10.1016/j.colsurfb.2023.113136Colloids and Surfaces B: Biointerfaces 222 (2023): 113136
ISSN0927-7765 (print); 1873-4367 (online)
Funded byThis work was supported by the grant Ayudas a Proyectos de I+D para Jovenes Doctores de la Universidad Autonoma de Madrid 2021 (SI3/PJI/2021–00216) supported by Comunidad de Madrid and Universidad Autonoma de Madrid to M.H-P. Also, M.H-P acknowledges funding from the Spanish Ministry of Science and Innovation (TED2021–129937B-I00). Grant PID2019–104098GB-I00/AEI/ 10.13039/501100011033, co-funded by the Spanish State Research Agency and the European Regional Development Fund to C.S.M. The CNB-CSIC was further supported by a Spanish State Research Agency Severo Ochoa Excellence grant (SEV 2017–0712). C.S.M is a member of the CSIC funded consortium LifeHub (CSIC grant number: 202120E47). REACT-EU funding by Comunidad Autonoma de Madrid is also acknowledged. M.M.S acknowledges funding from MCIN/AEI /10.13039/501100011033 (PID2020–112770RB-C22). P.J.P acknowledges projects FIS2017–89549-R; and FIS2017–90701-REDT. P.J.P also acknowledges the Human Frontiers Science Program (HFSPO RGP0012/2018)
ProjectGobierno de España. TED2021-129937B-I00; Gobierno de España. PID2019-104098GB-I00
SubjectsAFM; Human adenovirus; Hydrophobic surfaces; Reovirus; Titanate-assisted organosilanization; Física
Rights© 2023 The Authors
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Studies of virus adsorption on surfaces with optimized properties have attracted a lot of interest, mainly due to the influence of the surface in the retention, orientation and stability of the viral capsids. Besides, viruses in whole or in parts can be used as cages or vectors in different areas, such as biomedicine and materials science. A key requirement for virus nanocage application is their physical properties, i.e. their mechanical response and the distribution of surface charge, which determine virus-substrate interactions and stability. In the present work we show two examples of viruses exhibiting strong surface interactions on homogeneous hydrophobic surfaces. The surfaces were prepared by titanate assisted organosilanization, a sol-gel spin coating process, followed by a mild annealing step. We show by surface and interface spectroscopies that the process allows trapping triethoxy-octylsilane (OCTS) molecules, exhibiting a hydrophobic alkane rich surface finishing. Furthermore, the surfaces remain flat and behave as more efficient sorptive surfaces for virus particles than mica or graphite (HOPG). Also, we determine by atomic force microscopy (AFM) the mechanical properties of two types of viruses (human adenovirus and reovirus) and compare the results obtained on the OCTS functionalized surfaces with those obtained on mica and HOPG. Finally, the TIPT+OCTS surfaces were validated as platforms for the morphological and mechanical characterization of virus particles by using adenovirus as initial model and using HOPG and mica as standard control surfaces. Then, the same characteristics were determined on reovirus using TIPT+OCTS and HOPG, as an original contribution to the catalogue of physical properties of viral particles
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Google Scholar:Sanz Calderón, Aida - Cantero Reviejo, Miguel - Pérez, Uxia - Ortega González, Paula - San Martín, Carmen - Pablo Gómez, Pedro José de - Manso Silván, Miguel - Hernando Pérez, Mercedes
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