Cumulus Cell DNA damage as an index of human oocyte competence
EntityUAM. Departamento de Biología
10.1007/s43032-021-00817-7Reproductive Sciences 29.11 (2022): 3194 - 3200
ISSN1933-7191 (print); 1933-7205 (online)
SubjectsChoriogonadotropin Alfa (Drug); Ganirelix; Human Menopausal Gonadotropin; Menopur 75; Recombinant Follitropin; Biología y Biomedicina / Biología
Rights© The Author(s) 2021
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
The determination of oocyte quality is crucial for achieving effective syngamy post-sperm injection and embryonic development. Cumulus cells (CCs) have been proposed as biomarkers of oocyte quality because of their close bio-dynamic relationship with the oocyte. To determine the quality of the oocyte, CCs were sampled during oocyte preparation for ICSI to determine a CC DNA fragmentation index (CCDFI) of each individual oocyte using a variant of the chromatin dispersion test. One hundred and thirty oocytes were selected and studied from two Spanish fertility clinics, 90 of which were fertilized and developed to embryos. Significant differences were found between the CCDFI of unfertilized and fertilized oocytes (p <.001) and between the CCDFI of embryos that were discarded and those that developed suitable for transfer or cryopreservation (p <.001). Oocyte quality was negatively correlated with CCDFI (Spearman’s rho = − 0.45; p <.001). Receiver operator characteristics curves (ROC) suggested that a cut-off value of 24% CCDFI was able to discriminate the capacity of the gametes to result in syngamy with a sensitivity and specificity of 75.6% and 65%, respectively. This cut-off supports the application of CCDFI as potential index for the evaluation of the reproductive potential of oocytes prior to fertilization
Google Scholar:Baratas, Alejandro - Gosalbez Berenguer, José Jaime - Casa, Moisés de la - Camacho, Silvia - Dorado‑Silva, Monica - Johnston, Stephen D. - Roy Barcelona, Rosa
This item appears in the following Collection(s)
Showing items related by title, author, creator and subject.