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dc.contributor.authorGuerra, Pablo
dc.contributor.authorGonzález-Alamos, María
dc.contributor.authorLlauró Portell, Aida
dc.contributor.authorCasañas, Arnau
dc.contributor.authorQuerol-Audí, Jordi
dc.contributor.authorPablo Gómez, Pedro José de 
dc.contributor.authorVerdaguer, Núria
dc.contributor.otherUAM. Departamento de Física de la Materia Condensadaes_ES
dc.date.accessioned2023-02-27T09:10:38Z
dc.date.available2023-02-27T09:10:38Z
dc.date.issued2022-02-11
dc.identifier.citationScience Advances 8.6 (2022): eabj7795es_ES
dc.identifier.issn2375-2548 (online)es_ES
dc.identifier.urihttp://hdl.handle.net/10486/706505
dc.description.abstractVaults are ubiquitous ribonucleoprotein particles involved in a diversity of cellular processes, with promising applications as nanodevices for delivery of multiple cargos. The vault shell is assembled by the symmetrical association of multiple copies of the major vault protein that, initially, generates half vaults. The pairwise, anti-parallel association of two half vaults produces whole vaults. Here, using a combination of vault recombinant reconstitution and structural techniques, we characterized the molecular determinants for the vault opening process. This process commences with a relaxation of the vault waist, causing the expansion of the inner cavity. Then, local disengagement of amino-terminal domains at the vault midsection seeds a conformational change that leads to the aperture, facilitating access to the inner cavity where cargo is hosted. These results inform a hitherto uncharacterized step of the vault cycle and will aid current engineering efforts leveraging vault for tailored cargo deliveryes_ES
dc.format.extent11 pag.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherAmerican Association for the Advancement of Sciencees_ES
dc.relation.ispartofScience Advanceses_ES
dc.rights© 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Sciencees_ES
dc.subject.otherCellular Processes_ES
dc.subject.otherInner Cavitieses_ES
dc.subject.otherMajor Vault Proteinses_ES
dc.subject.otherRibonucleoprotein Particleses_ES
dc.subject.otherNano-Deviceses_ES
dc.titleSymmetry disruption commits vault particles to disassemblyes_ES
dc.typearticlees_ES
dc.subject.ecienciaFísicaes_ES
dc.relation.publisherversionhttps://doi.org/10.1126/sciadv.abj7795es_ES
dc.identifier.doi10.1126/sciadv.abj7795es_ES
dc.identifier.publicationfirstpageeabj7795-1es_ES
dc.identifier.publicationissue6es_ES
dc.identifier.publicationlastpageeabj7795-11es_ES
dc.identifier.publicationvolume8es_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dc.rights.ccReconocimientoes_ES
dc.rights.accessRightsopenAccesses_ES
dc.facultadUAMFacultad de Cienciases_ES


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