TCFL5 deficiency impairs the pachytene to diplotene transition during spermatogenesis in the mouse
Entidad
UAM. Departamento de Biología MolecularEditor
Nature ResearchFecha de edición
2022-12-01Cita
10.1038/s41598-022-15167-w
Scientific Reports 12.1 (2022): 10956
ISSN
2045-2322 (online)DOI
10.1038/s41598-022-15167-wProyecto
Gobierno de España. SAF2016-75988-R; Gobierno de España. PID2019-104760RB-I00; Comunidad de Madrid. S2017/BMD-3671/INFLAMUNE-CMVersión del editor
https://doi.org/10.1038/s41598-022-15167-wMaterias
Cell Cycle Protein; Stag3 Protein, Mouse; Transcription Factor; Biología y Biomedicina / BiologíaDerechos
© Te Author(s) 2022Resumen
Spermatogenesis is a complex, multistep process during which spermatogonia give rise to spermatozoa. Transcription Factor Like 5 (TCFL5) is a transcription factor that has been described expressed during spermatogenesis. In order to decipher the role of TCFL5 during in vivo spermatogenesis, we generated two mouse models. Ubiquitous removal of TCFL5 generated by breeding TCFL5fl/fl with SOX2-Cre mice resulted in sterile males being unable to produce spermatozoa due to a dramatic alteration of the testis architecture presenting meiosis arrest and lack of spermatids. SYCP3, SYCP1 and H1T expression analysis showed that TCFL5 deficiency causes alterations during pachytene/diplotene transition resulting in a meiotic arrest in a diplotene-like stage. Even more, TCFL5 deficient pachytene showed alterations in the number of MLH1 foci and the condensation of the sexual body. In addition, tamoxifen-inducible TCFL5 knockout mice showed, besides meiosis phenotype, alterations in the spermatids elongation process resulting in aberrant spermatids. Furthermore, TCFL5 deficiency increased spermatogonia maintenance genes (Dalz, Sox2, and Dmrt1) but also increased meiosis genes (Syce1, Stag3, and Morc2a) suggesting that the synaptonemal complex forms well, but cannot separate and meiosis does not proceed. TCFL5 is able to bind to the promoter of Syce1, Stag3, Dmrt1, and Syce1 suggesting a direct control of their expression. In conclusion, TCFL5 plays an essential role in spermatogenesis progression being indispensable for meiosis resolution and spermatids maturation
Lista de ficheros
Google Scholar:Galán Martínez, Javier
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Berenguer López, Inés
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Maza Moreno, María del Carmen
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Stamatakis Andriani, Konstantinos
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Gironés Pujol, Nuria
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Fresno Escudero, Manuel
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