Tandem Repeat DNA provides many cytological markers for hybrid zone analysis in two subspecies of the grasshopper chorthippus parallelus
Entity
UAM. Departamento de BiologíaPublisher
MDPIDate
2023-02-03Citation
10.3390/genes14020397
Genes 14.2 (2023): 397
ISSN
2073-4425 (online)DOI
10.3390/genes14020397Project
Gobierno de España. PID2019-104952GB-I00; info:eu-repo/grantAgreement/EC/H2020/875732/EU//birdGRCEditor's Version
https://doi.org/10.3390/genes14020397Subjects
FISH; Chorthippus Parallelus; Repetitive DNA; Tandem Repeats; Hybrid Zone; Biología y Biomedicina / BiologíaRights
© 2023 by the authors. Licensee MDPI, Basel, SwitzerlandAbstract
Recent advances in next generation sequencing (NGS) have greatly increased our understanding of non-coding tandem repeat (TR) DNA. Here we show how TR DNA can be useful for the study of hybrid zones (HZ), as it serves as a marker to identify introgression in areas where two biological entities come in contact. We used Illumina libraries to analyse two subspecies of the grasshopper Chorthippus parallelus, which currently form a HZ in the Pyrenees. We retrieved a total of 152 TR sequences, and used fluorescent in situ hybridization (FISH) to map 77 families in purebred individuals from both subspecies. Our analysis revealed 50 TR families that could serve as markers for analysis of this HZ, using FISH. Differential TR bands were unevenly distributed between chromosomes and subspecies. Some of these TR families yielded FISH bands in only one of the subspecies, suggesting the amplification of these TR families after the geographic separation of the subspecies in the Pleistocene. Our cytological analysis of two TR markers along a transect of the Pyrenean hybrid zone showed asymmetrical introgression of one subspecies into the other, consistent with previous findings using other markers. These results demonstrate the reliability of TR-band markers for hybrid zone studies
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Google Scholar:Navarro-Domínguez, Beatriz
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Cabrero, Josefa
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López-León, María Dolores
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Ruiz-Ruano, Francisco J.
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Pita Domínguez, Miguel
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Bella Sombría, José Luis
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Camacho, Juan Pedro M.
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