Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
Entity
UAM. Departamento de Química Física AplicadaPublisher
MDPIDate
2022-01-23Citation
10.3390/foods11030298
Foods 11.3 (2022): 298
ISSN
2304-8158 (online)DOI
10.3390/foods11030298Editor's Version
https://doi.org/10.3390/foods11030298Subjects
Antioxidants; Enzymes; Immunomodulation; Response surface methodology; Terfezia claveryi; Tuber aestivum; Ciencia y Tecnología de AlimentosRights
© 2022 by the authorsAbstract
A PLE (pressurized liquid extraction) method was adjusted following a full-factorial experimental design to obtain bioactive-enriched fractions from Tuber aestivum and Terfezia claveryi. Temperature, time and solvent (water, ethanol and ethanol–water 1:1) parameters were investi-gated. The response variables investigated were: obtained yield and the levels of total carbohydrate (compounds, β-glucans, chitin, proteins, phenolic compounds and sterols). Principal component analysis indicated water solvent and high temperatures as more adequate parameters to extract polysaccharide-rich fractions (up to 68% of content), whereas ethanol was more suitable to extract fungal sterols (up to 12.5% of content). The fractions obtained at optimal conditions (16.7 MPa, 180◦ C, 30 min) were able to protect Caco2 cells from free radical exposure, acting as antioxidants, and were able to reduce secretion of pro-inflammatory cytokines in vitro: IL-6 (50%), and TNFα (80% only T. claveryi ethanol extract), as well as reduce high inhibitory activity (T. aestivum IC50: 9.44 mG/mL)
Files in this item
Google Scholar:Tejedor Calvo, Eva
-
García-Barreda, Sergi
-
Sánchez, Sergio
-
Morte, Asunción
-
Siles Sánchez, María de las Nieves
-
Soler Rivas, Cristina
-
Santoyo Díez, Susana
-
Marco, Pedro
This item appears in the following Collection(s)
Related items
Showing items related by title, author, creator and subject.