Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
EntityUAM. Departamento de Química Física Aplicada
10.3390/foods11030298Foods 11.3 (2022): 298
SubjectsAntioxidants; Enzymes; Immunomodulation; Response surface methodology; Terfezia claveryi; Tuber aestivum; Ciencia y Tecnología de Alimentos
Rights© 2022 by the authors
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
A PLE (pressurized liquid extraction) method was adjusted following a full-factorial experimental design to obtain bioactive-enriched fractions from Tuber aestivum and Terfezia claveryi. Temperature, time and solvent (water, ethanol and ethanol–water 1:1) parameters were investi-gated. The response variables investigated were: obtained yield and the levels of total carbohydrate (compounds, β-glucans, chitin, proteins, phenolic compounds and sterols). Principal component analysis indicated water solvent and high temperatures as more adequate parameters to extract polysaccharide-rich fractions (up to 68% of content), whereas ethanol was more suitable to extract fungal sterols (up to 12.5% of content). The fractions obtained at optimal conditions (16.7 MPa, 180◦ C, 30 min) were able to protect Caco2 cells from free radical exposure, acting as antioxidants, and were able to reduce secretion of pro-inflammatory cytokines in vitro: IL-6 (50%), and TNFα (80% only T. claveryi ethanol extract), as well as reduce high inhibitory activity (T. aestivum IC50: 9.44 mG/mL)
Google Scholar:Tejedor Calvo, Eva - García-Barreda, Sergi - Sánchez, Sergio - Morte, Asunción - Siles Sánchez, María de las Nieves - Soler Rivas, Cristina - Santoyo Díez, Susana - Marco, Pedro
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