Microwave-assisted acid hydrolysis vs. conventional hydrolysis to produce sapogenin-rich products from fenugreek extracts
EntityUAM. Departamento de Química Física Aplicada
10.3390/foods11131934Foods 11.13 (2022): 1934
Funded byThis research was funded by Ministerio de Ciencia e Innovación of Spain, grant number FORDISCOVERY PID2019-110183RB-C22/AEI/10.13039/501100011033 and by the Community of Madrid (Spain), grant number ALIBIRD2020-CM, S2018/BAA-4343
ProjectGobierno de España. PID2019-110183RB-C22; Comunidad de Madrid. P2018/BAA-4343- ALIBIRD2020-CM
SubjectsDiabetes Mellitus; 4-Hydroxyisoleucine; Diosgenin; Física; Química
Rights© 2022 by the authors
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
The acid hydrolysis of saponins is commonly performed by conventional heating to produce sapogenin-rich products of bioactive interest, but alternative hydrolysis methods and their impact on bioactivity have been unexplored. We compared the conventional method with microwave-assisted acid hydrolysis (MAAH) of a commercial saponin-rich extract from a typical saponin source, fenugreek, focusing on the study of temperature (100, 120, 130, 140, 150◦C) and time (10, 20, 30, 40 min) of hydrolysis. The impact of these factors was assayed on both the sapogenin yield and the bioactivity of the hydrolyzed products, specifically their antioxidant and lipase inhibitory activities. The highest sapogenin content (34 g/100 g extract) was achieved by MAAH at 140◦C and 30 min, which was higher than conventional hydrolysis at both reference conditions (100◦C, 60 min, 24.6 g/100 g extract) and comparative conditions (140◦C, 30 min, 17 g/100 g extract) (p < 0.001). Typical steroid artifacts from sapogenins were observed in very small amounts, regardless of the method of hydrolysis. Antioxidant activity of MAAH hydrolyzed extracts (around 80% DPPH inhibition) was barely affected by time and temperature, but pancreatic lipase inhibitory activity was higher (>65%) at lower MAAH temperature (<130◦C) and time (<30 min) of hydrolysis. MAAH is shown as a valid alternative to produce selective sapogenin-rich extracts from fenugreek with minor impact on their bioactivities, and whose magnitude can be modulated by the hydrolysis conditions
Google Scholar:Navarro del Hierro, Joaquín - Cantero Bahíllo, Emma - Fernández-Felipe, M. Teresa - Martín García, Diana
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