The Early Oxidative Stress Induced by Mercury and Cadmium Is Modulated by Ethylene in Medicago sativa Seedlings
Entity
UAM. Departamento de BiologíaDate
2023-03-01Citation
10.3390/antiox12030551
Antioxidants 12.3 (2023): 551
ISSN
2076-3921DOI
10.3390/antiox12030551Project
Gobierno de España. AGL2010-15151; Gobierno de España. AGL2014-53771-R; Gobierno de España. AGL2017-87591-RSubjects
alfalfa; cadmium; ethylene; heavy metals; mercury; oxidative stress; Biología y Biomedicina / BiologíaRights
© 2023 by the authorsAbstract
Cadmium (Cd) and mercury (Hg) are ubiquitous soil pollutants that promote the accumulation of reactive oxygen species, causing oxidative stress. Tolerance depends on signalling processes that activate different defence barriers, such as accumulation of small heat sock proteins (sHSPs), activation of antioxidant enzymes, and the synthesis of phytochelatins (PCs) from the fundamental antioxidant peptide glutathione (GSH), which is probably modulated by ethylene. We studied the early responses of alfalfa seedlings after short exposure (3, 6, and 24 h) to moderate to severe concentration of Cd and Hg (ranging from 3 to 30 μM), to characterize in detail several oxidative stress parameters and biothiol (i.e., GSH and PCs) accumulation, in combination with the ethylene signalling blocker 1-methylcyclopropene (1-MCP). Most changes occurred in roots of alfalfa, with strong induction of cellular oxidative stress, H2O2 generation, and a quick accumulation of sHSPs 17.6 and 17.7. Mercury caused the specific inhibition of glutathione reductase activity, while both metals led to the accumulation of PCs. These responses were attenuated in seedlings incubated with 1-MCP. Interestingly, 1-MCP also decreased the amount of PCs and homophytochelatins generated under metal stress, implying that the overall early response to metals was controlled at least partially by ethylene
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Google Scholar:Flores-Cáceres, María Laura
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Ortega Villasante, Cristina
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Carril, Pablo
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Sobrino Plata, Juan
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Hernández, Luis E.
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