Electrostatic screening, acidic pH and macromolecular crowding increase the self-assembly efficiency of the minute virus of mice capsid in vitro
EntityUAM. Departamento de Biología Molecular
10.3390/v15051054Viruses 15.5 (2023): 1054
Funded byThis research was funded by grants from MICINN/FEDER EU (Spain, RTI2018-096635-B-100 and PID2021-126973OB-I00) to M.G.M. and by an institutional grant from Fundación Ramón Areces
ProjectGobierno de España. RTI2018–096635-B-I00; Gobierno de España. PID2021-126973OB-I00
SubjectsMice; Static Electricity; Hydrogen-Ion Concentration; Virus Assembly; Biología y Biomedicina / Biología
Rights© 2023 by the authors
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
The hollow protein capsids from a number of different viruses are being considered for multiple biomedical or nanotechnological applications. In order to improve the applied potential of a given viral capsid as a nanocarrier or nanocontainer, specific conditions must be found for achieving its faithful and efficient assembly in vitro. The small size, adequate physical properties and specialized biological functions of the capsids of parvoviruses such as the minute virus of mice (MVM) make them excellent choices as nanocarriers and nanocontainers. In this study we analyzed the effects of protein concentration, macromolecular crowding, temperature, pH, ionic strength, or a combination of some of those variables on the fidelity and efficiency of self-assembly of the MVM capsid in vitro. The results revealed that the in vitro reassembly of the MVM capsid is an efficient and faithful process. Under some conditions, up to ~40% of the starting virus capsids were reassembled in vitro as free, non aggregated, correctly assembled particles. These results open up the possibility of encapsidating different compounds in VP2-only capsids of MVM during its reassembly in vitro, and encourage the use of virus-like particles of MVM as nanocontainers
Google Scholar:Fuertes Villadangos, Miguel Ángel - López Mateos, Diego - Valiente Martínez-Sicluna, Luis - Rodríguez-Huete, Alicia - Valbuena Jiménez, Alejandro - García Mateu, Mauricio
This item appears in the following Collection(s)
Showing items related by title, author, creator and subject.