Electrostatic screening, acidic pH and macromolecular crowding increase the self-assembly efficiency of the minute virus of mice capsid in vitro
Entity
UAM. Departamento de Biología MolecularPublisher
MDPIDate
2023-05-01Citation
10.3390/v15051054
Viruses 15.5 (2023): 1054
ISSN
1999-4915 (online)DOI
10.3390/v15051054Funded by
This research was funded by grants from MICINN/FEDER EU (Spain, RTI2018-096635-B-100 and PID2021-126973OB-I00) to M.G.M. and by an institutional grant from Fundación Ramón ArecesProject
Gobierno de España. RTI2018–096635-B-I00; Gobierno de España. PID2021-126973OB-I00Editor's Version
https://doi.org/10.3390/v15051054Subjects
Mice; Static Electricity; Hydrogen-Ion Concentration; Virus Assembly; Biología y Biomedicina / BiologíaRights
© 2023 by the authorsAbstract
The hollow protein capsids from a number of different viruses are being considered for multiple biomedical or nanotechnological applications. In order to improve the applied potential of a given viral capsid as a nanocarrier or nanocontainer, specific conditions must be found for achieving its faithful and efficient assembly in vitro. The small size, adequate physical properties and specialized biological functions of the capsids of parvoviruses such as the minute virus of mice (MVM) make them excellent choices as nanocarriers and nanocontainers. In this study we analyzed the effects of protein concentration, macromolecular crowding, temperature, pH, ionic strength, or a combination of some of those variables on the fidelity and efficiency of self-assembly of the MVM capsid in vitro. The results revealed that the in vitro reassembly of the MVM capsid is an efficient and faithful process. Under some conditions, up to ~40% of the starting virus capsids were reassembled in vitro as free, non aggregated, correctly assembled particles. These results open up the possibility of encapsidating different compounds in VP2-only capsids of MVM during its reassembly in vitro, and encourage the use of virus-like particles of MVM as nanocontainers
Files in this item
Google Scholar:Fuertes Villadangos, Miguel Ángel
-
López Mateos, Diego
-
Valiente Martínez-Sicluna, Luis
-
Rodríguez-Huete, Alicia
-
Valbuena Jiménez, Alejandro
-
García Mateu, Mauricio
This item appears in the following Collection(s)
Related items
Showing items related by title, author, creator and subject.