Free PCR virus detection via few-layer bismuthene and tetrahedral DNA nanostructured assemblies
Author
Gutiérrez Gálvez, Laura; García Fernández, Daniel; Barrio Redondo, Melisa del; Luna Estévez, Mónica I.; Torres Peña, Iñigo; Zamora Abanades, Félix Juan; Navío Bernabeu, Cristina; Milán Rois, Paula; Castellanos Molina, Milagros; Abreu, Melanie; Cantón, Rafael; Galán Montemayor, Juan Carlos; Somoza Calatrava, Alvaro; Miranda Soriano, Rodolfo; García Mendiola, Tania; Lorenzo Abad, EncarnaciónEntity
UAM. Departamento de Química Analítica y Análisis Instrumental; UAM. Departamento de Química InorgánicaPublisher
ElsevierDate
2023-11-14Citation
10.1016/j.talanta.2023.125405
Talanta 269.1 (2024): 125405
ISSN
0039-9140 (print); 1873-3573 (online)DOI
10.1016/j.talanta.2023.125405Funded by
PID2020-116728RB-I00, PID2020-116661RB-I00, PID2020-119352RB-I00, PDC2021-120782-C2, PID2022-138908NB-C31, CTQ2015-71955-REDT, S2018/NMT-4349Project
Gobierno de España. PID2020-116728RB-I00; Gobierno de España. PID2020-116661RB-I00; Gobierno de España. PID2020-119352RB-I00; Gobierno de España. PDC2021-120782-C21; Gobierno de España. PID2022-138908NB-C31; Gobierno de España. CTQ2015-71955-REDT; Comunidad de Madrid. S2018/NMT-4349/TRANSNANOAVANSENSEditor's Version
https://doi.org/10.1016/j.talanta.2023.125405Subjects
Biocatalysis; Electrochemiluminescence; SARS-CoV-2; DNA Biosensor; Few-Layer Bismuthene; Tetrahedral DNA Nanostructures; Medicina; QuímicaRights
© 2023 The AuthorsEsta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Abstract
In this work we describe a highly sensitive method based on a biocatalyzed electrochemiluminescence approach. The system combines, for the first time, the use of few-layer bismuthene (FLB) as a platform for the oriented immobilization of tetrahedral DNA nanostructures (TDNs) specifically designed and synthetized to detect a specific SARS-CoV-2 gene sequence. In one of its vertices, these TDNs contain a DNA capture probe of the open reading frame 1 ab (ORF1ab) of the virus, available for the biorecognition of the target DNA/RNA. At the other three vertices, there are thiol groups that enable the stable anchoring/binding to the FLB surface. This novel geometry/approach enables not only the binding of the TDNs to surfaces, but also the orientation of the capture probe in a direction normal to the bismuthine surface so that it is readily accessible for binding/recognition of the specific SARS-CoV-2 sequence. The analytical signal is based on the anodic electrochemiluminescence (ECL) intensity of luminol which, in turn, arises as a result of the reaction with H2O2, generated by the enzymatic reaction of glucose oxidation, catalyzed by the biocatalytic label avidin-glucose oxidase conjugate (Av-GOx), which acts as co-reactant in the electrochemiluminescent reaction. The method exhibits a limit of detection (LOD) of 4.31 aM and a wide linear range from 14.4 aM to 1.00 μM, and its applicability was confirmed by detecting SARS-CoV-2 in nasopharyngeal samples from COVID-19 patients without the need of any amplification process
Files in this item
Google Scholar:Gutiérrez Gálvez, Laura
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García Fernández, Daniel
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Barrio Redondo, Melisa del
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Luna Estévez, Mónica I.
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Torres Peña, Iñigo
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Zamora Abanades, Félix Juan
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Navío Bernabeu, Cristina
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Milán Rois, Paula
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Castellanos Molina, Milagros
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Abreu, Melanie
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Cantón, Rafael
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Galán Montemayor, Juan Carlos
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Somoza Calatrava, Alvaro
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Miranda Soriano, Rodolfo
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García Mendiola, Tania
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Lorenzo Abad, Encarnación
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