Mapping in vitro local material properties of intact and disrupted virions at high resolution using multi-harmonic atomic force microscopy
Entity
UAM. Departamento de Física de la Materia CondensadaPublisher
Royal Society of ChemistryDate
2013-06-07Citation
Nanoscale 5.11 (2013): 4729-4736ISSN
2040-3364 (print); 2040-3372 (online)Funded by
The authors thank the National Science Foundation Materials World Network grant DMR 1008189 “Probing in vitro Structure–Property–Function Relationships of Viruses at High-Resolution using Advanced Atomic Force Microscopy Methods” for financial support. Also, we acknowledge funding by grants from the Ministry of Science and Innovation of Spain, PIB2010US-00233, FIS2011-29493, Consolider CSD2010-00024, CAM project and the Comunidad de Madrid no. S2009/MAT-1467 and BFU2011- 29038Project
Gobierno de España. PIB2010US-00233; Gobierno de España. FIS2011-29493; Gobierno de España. CSD2010-00024; Comunidad de Madrid. S2009/MAT-1467; Gobierno de España. BFU2011-29038Editor's Version
https://doi.org/10.1039/c3nr34088kSubjects
Adhesion; Atomic force microscopy; Hydration; Medical nanotechnology; Stiffness; Viruses; Viscosity; FísicaRights
© 2013 The Royal Society of ChemistryAbstract
Understanding the relationships between viral material properties (stiffness, strength, charge density, adhesion, hydration, viscosity, etc.), structure (protein sub-units, genome, surface receptors, appendages), and functions (self-assembly, stability, disassembly, infection) is of significant importance in physical virology and nanomedicine. Conventional Atomic Force Microscopy (AFM) methods have measured a single physical property such as the stiffness of the entire virus from nano-indentation at a few points which severely limits the study of structure-property-function relationships. We present an in vitro dynamic AFM technique operating in the intermittent contact regime which synthesizes anharmonic Lorentz-force excited AFM cantilevers to map quantitatively at nanometer resolution the local electro-mechanical force gradient, adhesion, and hydration layer viscosity within individual 29 virions. Furthermore, the changes in material properties over the entire 29 virion provoked by the local disruption of its shell are studied, providing evidence of bacteriophage depressurization. The technique significantly generalizes recent multi-harmonic theory (A. Raman, et al., Nat. Nanotechnol., 2011, 6, 809-814) and enables high-resolution in vitro quantitative mapping of multiple material properties within weakly bonded viruses and nanoparticles with complex structure that otherwise cannot be observed using standard AFM techniques
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Google Scholar:Cartagena, Alexander
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Hernando-Pérez, Mercedes
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Carrascosa, José L.
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de Pablo, Pedro J.
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Raman, Arvind
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