Strengths and weaknesses of the aniline-blue method used to test mushroom (1→3)-β-D-glucans obtained by microwave-assisted extractions
Publisher
ElsevierDate
2019-08-01Citation
Carbohydrate Polymers 217 (2019): 135-143ISSN
0144-8617 (print); 1879-1344 (online)Funded by
This research was supported by national R+D program from the Spanish Ministry of Science and Innovation (Project AGL2014-56211-R) and the regional program from the Community of Madrid, Spain ( S2013/ABI-2728 ). The authors would also like to thank the Brazilian funding agencies CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) , CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and the Fundação Araucária .Project
Comunidad de Madrid. S2013/ABI-2728; Gobierno de España. AGL2014-56211-REditor's Version
https://doi.org/10.1016/j.carbpol.2019.04.051Subjects
(1→3)-β-D-glucans; Fluorimetric assay; MAE; Microwave-assisted extraction; Mushrooms; Polysaccharides; Ciencia y Tecnología de AlimentosRights
© 2019 Elsevier LtdEsta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Abstract
The parameters to extract polysaccharide-enriched fractions (PEF) from mushrooms using MAE (microwave-assisted extraction) were adjusted following a full factorial 3 2 experimental design. The highest yield and total carbohydrate values, using Lentinula edodes as model mushroom, were obtained at 180 °C and 30 min. Several mushroom species were submitted to MAE and their PEF yields ranged between 12.1–44.2%. (1→3)-β-Glucans determination using a conventional fluorimetric method changed depending on the standard utilized. NMR analyses of PEF indicated that the presence of other polysaccharides in the extracts or their specific folding, might impair the proper determination of (1→3) linkages by the fluorophore. Mushrooms from Cantharellales order contained (1→3)-β-glucans but they were not detected with the fluorimetric method. Therefore, although the method (after adjustments) was sensitive enough to detect their presence in many mushroom extracts, it cannot be used for all species and it is also not recommended for quantitative determinations
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Google Scholar:Gil-Ramírez, Alicia
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Smiderle, Fhernanda R.
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Morales Hernández, Diego
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Iacomini, Marcello
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Soler Rivas, Cristina
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