Interactions of human galectins with Trypanosoma cruzi: binding profile correlate with genetic clustering of lineages

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dc.contributor.author Pineda, M. A.
dc.contributor.author Pineda, M. A.
dc.contributor.author Corvo, L.
dc.contributor.author Soto, M.
dc.contributor.author Fresno, Manuel
dc.contributor.author Bonay, Pedro
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.date.accessioned 2015-06-23T11:25:18Z
dc.date.available 2015-06-23T11:25:18Z
dc.date.issued 2015-01-01
dc.identifier.citation Glycobiology 25.2 (2015): 197-210 en_US
dc.identifier.issn 0959-6658 (print) en_US
dc.identifier.issn 1460-2423 (online) en_US
dc.identifier.uri http://hdl.handle.net/10486/666983
dc.description This is a pre-copyedited, author-produced PDF of an article accepted for publication in Glycobiology following peer review. The version of record Pineda, M.A. et al. Interactions of human galectins with Trypanosoma cruzi: binding profile correlate with genetic clustering of lineages. Glycobiology 25.2 (2015): 197-210 is available online at: http://dx.doi.org/10.1093/glycob/cwu103 en_US
dc.description.abstract We report here the specific interaction between several members of the human galectin family with the three developmental stages of several genetic lineages of the protozoan parasite Trypanosoma cruzi. We provide data of specific and differential binding of human galectins-1, 3, 4, 7 and 8 to 14 strains of T. cruzi that belong to the six genetic lineages representing the genetic diversity of the parasite. It is shown that galectins preferentially bind forms present in the host, trypomastigotes and amastigotes, compared to the non-infective epimastigote present on the intestinal tract of the vector, reflecting the changes on glycosylation that occur during the metacyclogenesis and amastigogenesis process. Also, it is evidenced that galectin binding to the parasites promotes binding to the host cells and higher infection rates. In addition evidence is provided indicating that the intracellular amastigotes may take over the cytosolic pool of some galectins when released to the extracellular medium. Finaly, by applying unweighted pair group method analysis to the galectin binding profile to either cell-derived trypomastigotes or amastigotes we show that the differential binding profile by the host galectins to the six lineages resembles the clustering based in genetic data. Therefore, the differential binding profile for the six lineages could have implications in the immunopathology of Chagas’ disease, affecting the complex network of immune responses on which galectins mediate, thus providing linkage clues to the notion that different lineages may be related to different clnical forms of the disease. en_US
dc.description.sponsorship This work was supported by grants from the Fondo de Investigaciones Sanitarias-Ministerio de Sanidad (FIS-PI11/00033) to PB and (FIS-PI11/0095) to MS, and grant ChagasEpiNet (European VII framework Program) to MF. The financial support Network RICET from the FIS, Ministerio de Sanidad and Fundacion Ramon Areces is acknowledged es_ES
dc.format.extent 39 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Oxford University Press en_US
dc.relation.ispartof Glycobiology en_US
dc.rights © The Author 2014 en_US
dc.subject.other discrete typing units en_US
dc.subject.other galectin en_US
dc.subject.other host cell adhesion en_US
dc.subject.other Trypanosoma cruzi en_US
dc.title Interactions of human galectins with Trypanosoma cruzi: binding profile correlate with genetic clustering of lineages en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.date.embargoend 2016-01-01
dc.relation.publisherversion http://dx.doi.org/10.1093/glycob/cwu103 es_ES
dc.identifier.doi 10.1093/glycob/cwu103 es_ES
dc.identifier.publicationfirstpage 197 es_ES
dc.identifier.publicationissue 2 es_ES
dc.identifier.publicationlastpage 210 es_ES
dc.identifier.publicationvolume 25 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/223034 es_ES
dc.relation.projectID Comunidad de Madrid. S2010/BMD-2332/INDISNET es_ES
dc.type.version info:eu-repo/semantics/acceptedVersion en
dc.rights.accessRights openAccess en
dc.authorUAM Bonay Miarons, Pedro (259060)
dc.authorUAM Bonay, Pedro


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