Trypanosoma cruzi strains cause different myocarditis patterns in infected mice

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dc.contributor.author Rodriguez, Hector O.
dc.contributor.author Guerrero, Néstor A.
dc.contributor.author Fortes, Alen
dc.contributor.author Santi-Rocca, Julien
dc.contributor.author Gironès, Núria
dc.contributor.author Fresno, Manuel
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.date.accessioned 2015-06-23T12:39:51Z
dc.date.available 2015-06-23T12:39:51Z
dc.date.issued 2014-07-11
dc.identifier.citation Acta Tropica 139 (2014): 57-66 es_ES
dc.identifier.issn 1873-706X (online) en_US
dc.identifier.issn 0001-706X (print) en_US
dc.identifier.uri http://hdl.handle.net/10486/666987
dc.description.abstract Aims. Chagas disease pathology is dependent on the infecting T. cruzi strain. However, the relationship between the extent and type of myocarditis caused by different T. cruzi strains in the acute and chronic phases of infection has not been studied in detail. To address this, we infected mice with three genetically distant T. cruzi strains as well as infected in vitro different cell types. Methods and Results. Parasitemia was detected in mice infected with the Y and VFRA strains, but not with the Sc43 strain; however, only the Y strain was lethal. When infected with VFRA, mice showed higher inflammation and parasitism in the heart than with Sc43 strain. Y and VFRA caused homogeneous pancarditis with inflammatory infiltrates along the epicardium, whereas Sc43 caused inflammation preferentially in the auricles in association with intracellular parasite localization. We observed intramyocardic perivasculitis in mice infected with the VFRA and Y strains, but not with Sc43, during the acute phase, which suggests that endothelial cells may be involved in heart colonization by these more virulent strains. In in vitro infection assays, the Y strain had the highest parasite-cell ratio in epithelial, macrophage and endothelial cell lines, but Y and VFRA strains were higher than Sc43 in cardiomyocytes. Conclusions. This study supports parasite variability as a cause for the diverse cardiac outcomes observed in Chagas disease, and suggests that endothelial cells could be involved in heart infection during the acute phase. en_US
dc.description.sponsorship This work was partially supported by grants from “Fondo de Investigaciones Sanitarias” (PS09/00538 and PI12/00289); “Ministerio de Ciencia e Innovación” (SAF2010-18733); The European Union (ChagasEpiNet); “Comunidad de Madrid” S2010/BMD-2332; RED RECAVA RD06/0014/1013;RED RICET RD12/0018/004 and an institutional grant from “Fundación Ramon Areces” en_US
dc.format.extent 25 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Elselvier Science BV en_US
dc.relation.ispartof Acta Tropica en_US
dc.subject.other Chagas disease es_ES
dc.subject.other Myocarditis en_US
dc.subject.other T. cruzi en_US
dc.title Trypanosoma cruzi strains cause different myocarditis patterns in infected mice en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.date.embargoend 2015-11-01
dc.relation.publisherversion http://dx.doi.org/10.1016/j.actatropica.2014.07.005 es_ES
dc.identifier.doi 10.1016/j.actatropica.2014.07.005 es_ES
dc.identifier.publicationfirstpage 57 es_ES
dc.identifier.publicationlastpage 66 es_ES
dc.identifier.publicationvolume 139 es_ES
dc.relation.projectID Comunidad de Madrid. S2010/BMD-2332/INDISNET es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/223034 es_ES
dc.type.version info:eu-repo/semantics/acceptedVersion en
dc.rights.cc Reconocimiento – NoComercial – SinObraDerivada es_ES
dc.rights.accessRights openAccess en
dc.authorUAM Girones Pujol, Nuria (261139)
dc.authorUAM Santi-Rocca , Julien (264370)


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