The leukocyte activation receptor CD69 controls T cell differentiation through its interaction with galectin-1

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dc.contributor.author Fuente, Hortensia de la
dc.contributor.author Cruz-Adalia, Aranzazu
dc.contributor.author Martínez del Hoyo, Gloria
dc.contributor.author Cibrián-Vera, Danay
dc.contributor.author Bonay, Pedro
dc.contributor.author Pérez-Hernández, Daniel
dc.contributor.author Vázquez, Jesús
dc.contributor.author Navarro, Pilar
dc.contributor.author Gutierrez-Gallego, Ricardo
dc.contributor.author Ramirez-Huesca, Marta
dc.contributor.author Martín, Pilar
dc.contributor.author Sánchez-Madrid, Francisco
dc.contributor.other UAM. Departamento de Medicina es_ES
dc.date.accessioned 2015-07-09T11:15:45Z
dc.date.available 2015-07-09T11:15:45Z
dc.date.issued 2014-01-01
dc.identifier.citation Molecular and Cellular Biology 34.13 (2014): 2479–2487 en_US
dc.identifier.issn 0270-7306 (print) en_US
dc.identifier.issn 1098-5549 (online) en_US
dc.identifier.uri http://hdl.handle.net/10486/667325
dc.description.abstract CD69 is involved in immune cell homeostasis, regulating the T cell-mediated immune response through the control of Th17 cell differentiation. However, natural ligands for CD69 have not yet been described. Using recombinant fusion proteins containing the extracellular domain of CD69, we have detected the presence of a ligand(s) for CD69 on human dendritic cells (DCs). Pulldown followed by mass spectrometry analyses of CD69-binding moieties on DCs identified galectin-1 as a CD69 counterreceptor. Surface plasmon resonance and anti-CD69 blocking analyses demonstrated a direct and specific interaction between CD69 and galectin-1 that was carbohydrate dependent. Functional assays with both human and mouse T cells demonstrated the role of CD69 in the negative effect of galectin-1 on Th17 differentiation. Our findings identify CD69 and galectin-1 to be a novel regulatory receptor-ligand pair that modulates Th17 effector cell differentiation and function en_US
dc.description.sponsorship This work was funded by grants SAF2011-25834 and ERC-2011AdG 294340-GENTRIS to F.S.-M., RECAVA RD06/0014 from the Fondo de Investigaciones Sanitarias to J.V. and F.S.-M., and INDISNET 01592006 from the Comunidad de Madrid to F.S.-M. and P.M. and by grants from the Ministerio de Economia y Competitividad (PI11/01562 to P.N.) and the Generalitat de Catalunya-AGAUR (2009SGR1409 to P.N.). The Ministry of Science and Innovation and the Pro-CNIC Foundation support CNIC en_US
dc.format.extent 9 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher American Society for Microbiology en_US
dc.relation.ispartof Molecular and Cellular Biology en_US
dc.rights © 2014, American Society for Microbiology en_US
dc.subject.other CD69 en_US
dc.subject.other Cells en_US
dc.subject.other Interaction with Galectin-1 en_US
dc.title The leukocyte activation receptor CD69 controls T cell differentiation through its interaction with galectin-1 en_US
dc.type article en
dc.subject.eciencia Medicina es_ES
dc.relation.publisherversion http://dx.doi.org/:10.1128/MCB.00348-14 es_ES
dc.identifier.doi 10.1128/MCB.00348-14 es_ES
dc.identifier.publicationfirstpage 2479 es_ES
dc.identifier.publicationissue 13 es_ES
dc.identifier.publicationlastpage 2487 es_ES
dc.identifier.publicationvolume 34 es_ES
dc.relation.projectID Comunidad de Madrid. S2010/BMD-2332/INDISNET es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.accessRights openAccess en
dc.authorUAM Bonay Miarons, Pedro (259060)
dc.authorUAM Bonay, Pedro
dc.authorUAM Sánchez Madrid, Francisco (259404)


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