MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration

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dc.contributor.author Aranda, Juan F.
dc.contributor.author Reglero-Real, Natalia
dc.contributor.author Kremer, Leonor
dc.contributor.author Marcos-Ramiro, Beatriz
dc.contributor.author Ruiz-Sáenz, Ana
dc.contributor.author Calvo, María
dc.contributor.author Enrich, Carlos
dc.contributor.author Correas, Isabel
dc.contributor.author Millán, Jaime
dc.contributor.author Alonso, Miguel A.
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.date.accessioned 2015-07-22T11:28:16Z
dc.date.available 2015-07-22T11:28:16Z
dc.date.issued 2011-04-15
dc.identifier.citation Molecular Biology of the Cell 22.8 (2011): 1252-1262 en_US
dc.identifier.issn 1059-1524 (print) es_ES
dc.identifier.issn 1939-4586 (online) es_ES
dc.identifier.uri http://hdl.handle.net/10486/667515
dc.description Copyright (2011) American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Molecular Biology of the Cell 22.8 (2011): 1252-1262 and may be found at http://www .molbiolcell.org/cgi/doi/10.1091/mbc.E10-11-0910 en_US
dc.description.abstract Membrane organization into condensed domains or rafts provides molecular platforms for selective recruitment of proteins. Cell migration is a general process that requires spatiotemporal targeting of Rac1 to membrane rafts. The protein machinery responsible for making rafts competent to recruit Rac1 remains elusive. Some members of the MAL family of proteins are involved in specialized processes dependent on this type of membrane. Because condensed membrane domains are a general feature of the plasma membrane of all mammalian cells, we hypothesized that MAL family members with ubiquitous expression and plasma membrane distribution could be involved in the organization of membranes for cell migration. We show that myeloid-associated differentiation marker (MYADM), a protein with unique features within the MAL family, colocalizes with Rac1 in membrane protrusions at the cell surface and distributes in condensed membranes. MYADM knockdown (KD) cells had altered membrane condensation and showed deficient incorporation of Rac1 to membrane raft fractions and, similar to Rac1 KD cells, exhibited reduced cell spreading and migration. Results of rescue-of-function experiments by expression of MYADM or active Rac1L61 in cells knocked down for Rac1 or MYADM, respectively, are consistent with the idea that MYADM and Rac1 act on parallel pathways that lead to similar functional outcomes en_US
dc.description.sponsorship This work was supported by grants BFU2009–07886 (to M.A.A.), SAF-2008–01936 and SAF2008–01158-E (to J.M.), BFU2008–02460 (to I.C.), BFU2009–10335 (to C.E.), CONSOLIDER COAT CSD2009–00016 (to M.A.A. and C.E.), all grants from the Ministerio de Ciencia e Innovación, and grants GEN-0166/2006 from the Comunidad de Madrid (to I.C.), CSIC-200920I016 from Consejo Superior de Investigaciones Científicas (to L.K.), and PI040236 from Fundació Marató TV3 (to C.E.) en_US
dc.format.extent 11 pag. en
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher The American Society of Cell Biology en_US
dc.relation.ispartof Molecular Biology of the Cell en_US
dc.rights © 2011 Aranda et al. en_US
dc.subject.other Antigens en_US
dc.subject.other Cell Membrane en_US
dc.subject.other Electroporation en_US
dc.subject.other Transfection en_US
dc.title MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.relation.publisherversion http://dx.doi.org/10.1091/mbc.E10-11-0910 es_ES
dc.identifier.doi 10.1091/mbc.E10-11-0910 es_ES
dc.identifier.publicationfirstpage 1252 es_ES
dc.identifier.publicationissue 8 es_ES
dc.identifier.publicationlastpage 1262 es_ES
dc.identifier.publicationvolume 22 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.cc Reconocimiento – NoComercial – CompartirIgual es_ES
dc.rights.accessRights openAccess en
dc.authorUAM Correas Hornero, María Isabel (260150)


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