Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI

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dc.contributor.author Tarin, Carlos
dc.contributor.author Carril, Monica
dc.contributor.author Martin-Ventura, Jose Luis
dc.contributor.author Markuerkiaga, Irati
dc.contributor.author Padro, Daniel
dc.contributor.author Llamas-Granda, Patricia
dc.contributor.author Moreno, Juan Antonio
dc.contributor.author García, Isabel
dc.contributor.author Genicio, Nuria
dc.contributor.author Plaza-Garcia, Sandra
dc.contributor.author Blanco-Colio, Luis Miguel
dc.contributor.author Penades, Soledad
dc.contributor.author Egido, Jesus
dc.contributor.other UAM. Departamento de Medicina es_ES
dc.contributor.other Instituto de Investigación Sanitaria Fundación Jiménez Díaz (ISS-FJD) es_ES
dc.date.accessioned 2016-08-02T14:46:16Z
dc.date.available 2016-08-02T14:46:16Z
dc.date.issued 2015-11-30
dc.identifier.citation Scientific Reports 5 (2015): 1713517135 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10486/672360
dc.description.abstract CD163 is a membrane receptor expressed by macrophage lineage. Studies performed in atherosclerosis have shown that CD163 expression is increased at inflammatory sites, pointing at the presence of intraplaque hemorrhagic sites or asymptomatic plaques. Hence, imaging of CD163 expressing macrophages is an interesting strategy in order to detect atherosclerotic plaques. We have prepared a targeted probe based on gold-coated iron oxide nanoparticles vectorized with an anti-CD163 antibody for the specific detection of CD163 by MRI. Firstly, the specificity of the targeted probe was validated in vitro by incubation of the probe with CD163(+) or (−) macrophages. The probe was able to selectively detect CD163(+) macrophages both in human and murine cells. Subsequently, the targeted probe was injected in 16 weeks old apoE deficient mice developing atherosclerotic lesions and the pararenal abdominal aorta was imaged by MRI. The accumulation of probe in the site of interest increased over time and the signal intensity decreased significantly 48 hours after the injection. Hence, we have developed a highly sensitive targeted probe capable of detecting CD163-expressing macrophages that could provide useful information about the state of the atheromatous lesions en_US
dc.description.sponsorship This work was funded by Spanish Government through a Plan Nacional (CTQ2011–27268), FEDER funds through the Fondo de Investigación Sanitaria (PI10/00072, PI13/00051, PI13/00395, PI13/00802, PI14/00883 and PI14/00386), CIBERDEM group, RETICS RD12/0042/0038, Programa Miguel Servet (CP10/00479) and cvREMOD CENIT project (CEN-20091044), the Basque Government through Etortek 2011 (IE11–301), and Fundacion Lilly, Spanish Society of Atherosclerosis, Spanish Society of Nephrology and Fundacion Renal Iñigo Alvarez de Toledo en_US
dc.format.extent 9 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Nature Publishing Group en_US
dc.relation.ispartof Scientific Reports en_US
dc.subject.other Atherosclerosis en_US
dc.subject.other CD163 es_ES
dc.subject.other Sensitive targeted en_US
dc.subject.other Information en_US
dc.subject.other Atheromatous lesions en_US
dc.title Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI en_US
dc.type article en
dc.subject.eciencia Medicina es_ES
dc.relation.publisherversion http://dx.doi.org/10.1038/srep17135 en
dc.identifier.doi 10.1038/srep17135 es_ES
dc.identifier.publicationfirstpage 17135-1 es_ES
dc.identifier.publicationlastpage 17135-9 es_ES
dc.identifier.publicationvolume 5 es_ES
dc.relation.projectID Gobierno de España. CTQ2011–27268 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.cc Reconocimiento es_ES
dc.rights.accessRights openAccess en


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