Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles

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Show simple item record Vadivelu, Raja K. Ooi, Chin H. Yao, Rebecca Qing Tello Velasquez, Johana Pastrana, Erika Diaz-Nido, Javier Lim, Filip Ekberg, Jenny A K Nguyen, Nam Trung St John, James A.
dc.contributor.other UAM. Departamento de Biología Molecular es_ES 2016-09-09T08:54:01Z 2016-09-09T08:54:01Z 2015-10-14
dc.identifier.citation Scientific Reports 5 (2015): 15083 en_US
dc.identifier.issn 2045-2322 es_ES
dc.description.abstract We describe a novel protocol for three-dimensional culturing of olfactory ensheathing cells (OECs), which can be used to understand how OECs interact with other cells in three dimensions. Transplantation of OECs is being trialled for repair of the paralysed spinal cord, with promising but variable results and thus the therapy needs improving. To date, studies of OEC behaviour in a multicellular environment have been hampered by the lack of suitable three-dimensional cell culture models. Here, we exploit the floating liquid marble, a liquid droplet coated with hydrophobic powder and placed on a liquid bath. The presence of the liquid bath increases the humidity and minimises the effect of evaporation. Floating liquid marbles allow the OECs to freely associate and interact to produce OEC spheroids with uniform shapes and sizes. In contrast, a sessile liquid marble on a solid surface suffers from evaporation and the cells aggregate with irregular shapes. We used floating liquid marbles to co-culture OECs with Schwann cells and astrocytes which formed natural structures without the confines of gels or bounding layers. This protocol can be used to determine how OECs and other cell types associate and interact while forming complex cell structures en_US
dc.description.sponsorship JSJ was funded by a grant from the Perry Cross Spinal Research Foundation; NTN was funded from Griffith University through a start-up grant and a grant from the Griffith University Research Infrastructure Program; JAK was funded by an Australian Research Council Discovery Grant DP150104495; JT was funded by an Eskitis Institute scholarship; CO was funded by a Griffith Sciences scholarship; RV was funded by a Griffith University International Postgraduate Research Scholarship en_US
dc.format.extent 12 pag. en
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Nature Publishing Group en_US
dc.relation.ispartof Scientific Reports en_US
dc.subject.other Olfactory ensheathing cells (OECs) en_US
dc.subject.other Three-dimensional en_US
dc.subject.other Multiple spheroid model en_US
dc.subject.other Liquid marbles en_US
dc.title Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.relation.publisherversion es_ES
dc.identifier.doi 10.1038/srep15083 es_ES
dc.identifier.publicationfirstpage 15083-1 es_ES
dc.identifier.publicationlastpage 15083-12 es_ES
dc.identifier.publicationvolume 5 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.accessRights openAccess en
dc.authorUAM Lim , Filip (260854)

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