The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation

Biblos-e Archivo/Manakin Repository

Show simple item record

dc.contributor.author Reales, E.
dc.contributor.author Bernabé-Rubio, M.
dc.contributor.author Casares-Arias, J.
dc.contributor.author Rentero, C.
dc.contributor.author Fernández-Barrera, J.
dc.contributor.author Rangel Sánchez, Laura
dc.contributor.author Correas, Isabel
dc.contributor.author Enrich, C.
dc.contributor.author Andrés, G.
dc.contributor.author Alonso, M.A.
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.date.accessioned 2016-12-05T16:38:26Z
dc.date.available 2016-12-05T16:38:26Z
dc.date.issued 2015-04-30
dc.identifier.citation Journal of Cell Science 128.12 (2015): 2261-2270 en_US
dc.identifier.issn 0021-9533 (print) es_ES
dc.identifier.issn 1477-9137 (online) es_ES
dc.identifier.uri http://hdl.handle.net/10486/675808
dc.description.abstract The base of the primary cilium contains a zone of condensed membranes whose importance is not known. Here, we have studied the involvement of MAL, a tetraspanning protein that exclusively partitions into condensed membrane fractions, in the condensation of membranes at the ciliary base and investigated the importance of these membranes in primary cilium formation. We show that MAL accumulates at the ciliary base of epithelial MDCK cells. Knockdown of MAL expression resulted in a drastic reduction in the condensation of membranes at the ciliary base, the percentage of ciliated cells and the length of the cilia, but did not affect the docking of the centrosome to the plasma membrane or produce missorting of proteins to the pericentriolar zone or to the membrane of the remaining cilia. Rab8 (for which there are two isoforms, Rab8A and Rab8b), IFT88 and IFT20, which are important components of the machinery of ciliary growth, were recruited normally to the ciliary base of MAL-knockdown cells but were unable to elongate the primary cilium correctly. MAL, therefore, is crucial for the proper condensation of membranes at the ciliary base, which is required for efficient primary cilium extension en_US
dc.description.sponsorship This work was supported by the Ministerio de Economıa y Competitividad, Spain [grant numbers BFU2012-32532 and CONSOLIDER COAT CSD2009-00016 to M. A.A.]. G.A. was supported by the Amarouto Program for senior researchers from the Comunidad Autónoma de Madrid en_US
dc.format.extent 10 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Company of Biologists Ltd en_US
dc.relation.ispartof Journal of Cell Science en_US
dc.rights © 2015. Published by The Company of Biologists Ltd. en_US
dc.subject.other Centrosome en_US
dc.subject.other Condensed membranes en_US
dc.subject.other MAL en_US
dc.subject.other Primary ciliogenesis en_US
dc.title The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.relation.publisherversion http://dx.doi.org/10.1242/jcs.164970 es_ES
dc.identifier.doi 10.1242/jcs.164970 es_ES
dc.identifier.publicationfirstpage 2261 es_ES
dc.identifier.publicationissue 12 es_ES
dc.identifier.publicationlastpage 2270 es_ES
dc.identifier.publicationvolume 128 es_ES
dc.relation.projectID Gobierno de España. BFU2012-32532 es_ES
dc.relation.projectID Gobierno de España. CSD2009-00016 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.accessRights openAccess en
dc.authorUAM Correas Hornero, María Isabel (260150)


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record