Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

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dc.contributor.author Ramos-Gómez, M.
dc.contributor.author Seiz, E.G.
dc.contributor.author Martínez-Serrano, Alberto
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.date.accessioned 2017-01-23T16:15:24Z
dc.date.available 2017-01-23T16:15:24Z
dc.date.issued 2015-03-05
dc.identifier.citation Journal of Nanobiotechnology 13.1 (2015): 20 en_US
dc.identifier.issn 1477-3155 (print) es_ES
dc.identifier.uri http://hdl.handle.net/10486/676519
dc.description.abstract Background: Magnetic resonance imaging is the ideal modality for non-invasive in vivo cell tracking allowing for longitudinal studies over time. Cells labeled with superparamagnetic iron oxide nanoparticles have been shown to induce sufficient contrast for in vivo magnetic resonance imaging enabling the in vivo analysis of the final location of the transplanted cells. For magnetic nanoparticles to be useful, a high internalization efficiency of the particles is required without compromising cell function, as well as validation of the magnetic nanoparticles behaviour inside the cells. Results: In this work, we report the development, optimization and validation of an efficient procedure to label human neural stem cells with commercial nanoparticles in the absence of transfection agents. Magnetic nanoparticles used here do not affect cell viability, cell morphology, cell differentiation or cell cycle dynamics. Moreover, human neural stem cells progeny labeled with magnetic nanoparticles are easily and non-invasively detected long time after transplantation in a rat model of Parkinson's disease (up to 5 months post-grafting) by magnetic resonance imaging. Conclusions: These findings support the use of commercial MNPs to track cells for short- and mid-term periods after transplantation for studies of brain cell replacement therapy. Nevertheless, long-term MR images should be interpreted with caution due to the possibility that some MNPs may be expelled from the transplanted cells and internalized by host microglial cells en_US
dc.description.sponsorship This work was supported by grants from (to AM-S): Spanish Ministry of Economy and Competitiveness (SAF2010-17167), Comunidad Autónoma Madrid (S2011-BMD-2336), Instituto Salud Carlos III (RETICS TerCel, RD12/0019/ 0013). This work was also supported by an institutional grant from Fundación Ramón Areces to the Center of Molecular Biology Severo Ochoa. The authors gratefully acknowledge the financial support of the Reina Sofia Foundation and Comunidad Autónoma Madrid (S2010-BMD-2460) to MR-G en_US
dc.format.extent 15 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher BioMed Central Ltd. en_US
dc.relation.ispartof Journal of Nanobiotechnology en_US
dc.rights © 2015 Ramos-Gómez et al en_US
dc.subject.other Cell tracking en_US
dc.subject.other Magnetic nanoparticles en_US
dc.subject.other Magnetic resonance imaging en_US
dc.subject.other Neural stem cell en_US
dc.title Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.relation.publisherversion http://dx.doi.org/10.1186/s12951-015-0078-4 es_ES
dc.identifier.doi 10.1186/s12951-015-0078-4 es_ES
dc.identifier.publicationfirstpage 20 es_ES
dc.identifier.publicationissue 1 es_ES
dc.identifier.publicationlastpage 20 es_ES
dc.identifier.publicationvolume 13 es_ES
dc.relation.projectID Gobierno de España. SAF2010-17167 es_ES
dc.relation.projectID Comunidad de Madrid. S2011/BMD-2336/NEUROSTEM es_ES
dc.relation.projectID Comunidad de Madrid. S2010/BMD-2460/NEUROTEC es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.cc Reconocimiento es_ES
dc.rights.accessRights openAccess en

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