Genomic assemblies of newly sequenced Trypanosoma cruzi strains reveal new genomic expansion and greater complexity

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Show simple item record Callejas-Hernández, Francisco Rastrojo, Alberto Poveda, Cristina Gironès, Núria Fresno, Manuel
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.contributor.other Centro de Biología Molecular Severo Ochoa (CBMSO) es_ES
dc.contributor.other Instituto de Investigación Sanitaria Hospital Universitario de La Princesa (IIS-IP) es_ES 2018-12-11T18:07:04Z 2018-12-11T18:07:04Z 2018-10-02
dc.identifier.citation Scientific Reports 8.1 (2018) 14631 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.description.abstract Chagas disease is a complex illness caused by the protozoan Trypanosoma cruzi displaying highly diverse clinical outcomes. In this sense, the genome sequence elucidation and comparison between strains may lead to disease understanding. Here, two new T. cruzi strains, have been sequenced, Y using Illumina and Bug2148 using PacBio, assembled, analyzed and compared with the T. cruzi annotated genomes available to date. The assembly stats from the new sequences show effective improvement of T. cruzi genome over the actual ones. Such as, the largest contig assembled (1.3 Mb in Bug2148) in de novo attempts and the highest mean assembly coverage (71X for Y). Our analysis reveals a new genomic expansion and greater complexity for those multi-copy gene families related to infection process and disease development, such as Trans-sialidases, Mucins and Mucin Associated Surface Proteins, among others. On one side, we demonstrate that multi-copy gene families are located near telomeric regions of the “chromosome-like” 1.3 Mb contig assembled of Bug2148, where they likely suffer high evolutive pressure. On the other hand, we identified several strain-specific single copy genes that might help to understand the differences in infectivity and physiology among strains. In summary, our results indicate that T. cruzi has a complex genomic architecture that may have promoted its evolution. es_ES
dc.description.sponsorship This work was supported by the “Consejo Nacional de Ciencia y Tecnología” (CONACYT, México) through the FC-H Ph.D. studentship number 411595 and the “Consejo de Ciencia, Tecnología e Innovación de Hidalgo (CITNOVA, México);; “Ministerio de Economía y competitividad” (SAF2015-63868-R (MINECO/FEDER) to N.G., SAF2016-75988-R (MINECO/FEDER) to M.F.); “Red de Investigación de Centros de Enfermedades Tropicales” (RICET RD12/0018/0004 to M.F.); European Union (HEALTH-FE-2008-22303, ChagasEpiNet to M.F.); Comunidad de Madrid (S-2010/BMD-2332 to M.F.); and Institutional grants from “Fundación Ramón Areces” and “Banco de Santander” es_ES
dc.format.extent 13 pag. es_ES
dc.format.mimetype application/pdf es_ES
dc.language.iso eng es_ES
dc.publisher Nature Research (part of Springer Nature) es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights © The Author(s) 2018 es_ES
dc.subject.other Trypanosoma cruzi es_ES
dc.subject.other New genomic es_ES
dc.subject.other Expansion and greater complexity es_ES
dc.subject.other Strains es_ES
dc.title Genomic assemblies of newly sequenced Trypanosoma cruzi strains reveal new genomic expansion and greater complexity es_ES
dc.type article es_ES
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.relation.publisherversion es_ES
dc.identifier.doi 10.1038/s41598-018-32877-2 es_ES
dc.identifier.publicationfirstpage 14631-1 es_ES
dc.identifier.publicationissue 1 es_ES
dc.identifier.publicationlastpage 14631-13 es_ES
dc.identifier.publicationvolume 8 es_ES
dc.relation.projectID Gobierno de España. SAF2015-63868-R es_ES
dc.relation.projectID Gobierno de España. SAF2016-75988-R es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/22303 es_ES
dc.relation.projectID Comunidad de Madrid. S-2010/BMD-2332/INDISNET es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES Reconocimiento es_ES
dc.rights.accessRights openAccess es_ES
dc.authorUAM Girones Pujol, Nuria (261139)

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