Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion

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Show simple item record Hurtado-Gallego, J. Martín-Betancor, K. Rodea Palomares, Ismael Leganés, Francisco Rosal, R. Fernández-Piñas, Francisca
dc.contributor.other UAM. Departamento de Biología es_ES 2019-04-12T09:55:40Z 2019-04-12T09:55:40Z 2018-03-03
dc.identifier.citation Chemosphere 201 (2018): 772-779 en_US
dc.identifier.issn 1879-1298 (online) en_US
dc.identifier.issn 0045-6535 (print) en_US
dc.description This Accepted Manuscript is available for reuse under a CC BY-NC-ND 3.0 licence after the 24 month embargo period provided that all the terms of the licence are adhered to en_US
dc.description.abstract Ltd This work describes the construction of two novel self-luminescent bioreporter strains of the cyanobacterium Nostoc sp. PCC 7120 by fusing the promoter region of the sodA and sodB genes (encoding the superoxide dismutases MnSod and FeSod, respectively) to luxCDABE from Photorhabdus luminescens aimed at detecting pollutants that generate reactive oxygen species (ROS), particularly O2−. Bioreporters were tested against methyl viologen (MV) as the inducer of superoxide anion (O2−). Both bioreporters were specific for O2− and Limits of detection (LODs) and Maximum Permissive Concentrations (MPCs) were calculated: Nostoc sp. PCC 7120 pBG2154 (sodA) had a range of detection from 400 to 1000 pM of MV and for Nostoc sp. PCC 7120 pBG2165 (sodB) the range of detection was from 500 to 1800 pM of MV after 5 h-exposure. To further validate the bioreporters, they were tested with the emerging pollutant Triclosan which induced bioluminescence in both strains. Furthermore, the bioreporters performance was tested in two real environmental samples with different water matrix complexity, spiked with MV. Both bioreporters were induced by O2− in these environmental samples. In the case of the river water sample, the amount of bioavailable MV as calculated from the bioreporters output was similar to that nominally added. For the waste water sample, the bioavailable MV concentration detected by the bioreporters was one order of magnitude lower than nominal. These differences could be due to MV complexation with organic matter and/or co-occurring organic contaminants. These results confirm their high sensitivity to O2− and their suitability to detect oxidative stress-generating pollutants in fresh-waters en_US
dc.description.sponsorship This research is supported by CTM2013-45775-C2-1-R and CTM2013-45775-C2-2-R grants for MINECO . JHG is working under FPI contract (MINECO-EU) en_US
dc.format.extent 16 pag. en_US
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Elsevier en_US
dc.relation.ispartof Chemosphere en_US
dc.rights © 2018 Elsevier Ltd en_US
dc.subject.other Bioluminescence en_US
dc.subject.other Cyanobacterial bioreporter en_US
dc.subject.other Fe-sod en_US
dc.subject.other Mn-sod en_US
dc.subject.other Superoxide anion en_US
dc.subject.other Superoxide dismutases en_US
dc.title Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.subject.eciencia Química es_ES 2020-03-03
dc.relation.publisherversion es_ES
dc.identifier.doi 10.1016/j.chemosphere.2018.03.012 es_ES
dc.identifier.publicationfirstpage 772 es_ES
dc.identifier.publicationlastpage 779 es_ES
dc.identifier.publicationvolume 201 es_ES
dc.relation.projectID Gobierno de España. CTM2013-45775-C2-1-R es_ES
dc.relation.projectID Gobierno de España. CTM2013-45775-C2-2-R es_ES
dc.type.version info:eu-repo/semantics/acceptedVersion en Reconocimiento – NoComercial – SinObraDerivada es_ES
dc.rights.accessRights embargoedAccess en
dc.authorUAM Fernández Piñas, Francisca (260189)
dc.authorUAM Leganés Nieto, Francisco (261828)
dc.authorUAM Rodea Palomares, Ismael Martín (263998)

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