Caveolin-1α regulates primary cilium length by controlling RhoA GTPase activity

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Show simple item record Rangel, Laura Bernabé-Rubio, Miguel Fernández-Barrera, Jaime Casares-Arias, Javier Millán, Jaime Alonso, Miguel A. Correas, Isabel
dc.contributor.other UAM. Departamento de Biología es_ES 2019-04-30T14:04:27Z 2019-04-30T14:04:27Z 2019-02-04
dc.identifier.citation Scientific Reports 9 (2019): 1116 en_US
dc.identifier.issn 2045-2322 es_ES
dc.description.abstract The primary cilium is a single non-motile protrusion of the plasma membrane of most types of mammalian cell. The structure, length and function of the primary cilium must be tightly controlled because their dysfunction is associated with disease. Caveolin 1 (Cav1), which is best known as a component of membrane invaginations called caveolae, is also present in non-caveolar membrane domains whose function is beginning to be understood. We show that silencing of α and β Cav1 isoforms in different cell lines increases ciliary length regardless of the route of primary ciliogenesis. The sole expression of Cav1α, which is distributed at the apical membrane, restores normal cilium size in Cav1 KO MDCK cells. Cells KO for only Cav1α, which also show long cilia, have a disrupted actin cytoskeleton and reduced RhoA GTPase activity at the apical membrane, and a greater accumulation of Rab11 vesicles at the centrosome. Subsequent experiments showed that DIA1 and ROCK help regulate ciliary length. Since MDCK cells lack apical caveolae, our results imply that non-caveolar apical Cav1α is an important regulator of ciliary length, exerting its effect via RhoA and its effectors, ROCK and DIA1 en_US
dc.description.sponsorship This work was supported by grants B2017/BMD-3817 from the Comunidad de Madrid and BFU2015-67266-R from the Spanish Ministerio de Economía y Competitividad/Fondo Europeo de Desarrollo Regional (MINECO/FEDER) to IC and MAA, respectively. We also acknowledge the Micro and Nanofabrication Laboratory of the Instituto de Micro y Nanotecnología, which is funded from the Comunidad de Madrid (project SpaceTec, S2013/ICE2822), MINECO (project CSIC13-4E-1794) and EU (FEDER, FSE), for invaluable help on SEM. Contracts from the Ministerio de Educación, Cultura y Deporte (JF-B, JC-A) and the MINECO (MB-R) are also acknowledged en_US
dc.format.extent 16 págs. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Nature Research en_US
dc.relation.ispartof Scientific Reports en_US
dc.rights © 2019, The Author(s) en_US
dc.subject.other cilia en_US
dc.subject.other flagella en_US
dc.subject.other intraflagellar transport en_US
dc.title Caveolin-1α regulates primary cilium length by controlling RhoA GTPase activity en_US
dc.type article en_US
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.relation.publisherversion es_ES
dc.identifier.doi 10.1038/s41598-018-38020-5 es_ES
dc.identifier.publicationfirstpage 1116-1 es_ES
dc.identifier.publicationissue 9 es_ES
dc.identifier.publicationlastpage 1116-16 es_ES
dc.relation.projectID Gobierno de España. CSIC13-4E-1794 es_ES
dc.relation.projectID Comunidad de Madrid. S2013/ICE-2822/SPACETEC-CM es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en Reconocimiento es_ES
dc.rights.accessRights openAccess en_US
dc.authorUAM Millan Martínez, Jaime (264139)
dc.authorUAM Correas Hornero, María Isabel (260150)

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