Anti-inflammatory and antioxidant activities from the basolateral fraction of Caco-2 cells exposed to a rosmarinic acid enriched extract
Entity
UAM. Departamento de Química Física AplicadaPublisher
American Chemical SocietyDate
2018-01-18Citation
10.1021/acs.jafc.7b06008
Journal of Agricultural and Food Chemistry 66.5 (2018): 1167-1174
ISSN
0021-8561 (print); 1520-5118 (online)DOI
10.1021/acs.jafc.7b06008Funded by
Financial support was received from the Spanish Government (FORCHRONIC Project AGL-2016-76736-C3-1-R) and Comunidad Autonoma de Madrid (ALIBIRD, Project P2013/ ABI2728)Project
Gobierno de España. AGL2016-76736-C3-1-REditor's Version
https://doi.org/10.1021/acs.jafc.7b06008Subjects
Anti-Inflammatory Activity; Antioxidant Activity; Rosmarinic Acid; In Vitro Digestion; Caco-2 Cells; QuímicaNote
“This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, © 2018 American Chemical Society after peer review and technical editing by the publisher. To acces final work see “Anti-inflammatory and antioxidant activities from the basolateral fraction of Caco-2 cells exposed to a rosmarinic acid enriched extract”, Journal of Agricultural and Food Chemistry 66.5 (2018): 1167-1174, https://doi.org/10.1021/acs.jafc.7b06008”Rights
© 2018 American Chemical SocietyAbstract
The potential use of Origanum majorana L. as a source of bioavailable phenolic compounds, specifically rosmarinic acid (RA), has been evaluated. Phenolic bioavailability was tested using an in vitro digestion process followed by a Caco-2 cellular model of intestinal absorption. The high-performance liquid chromatography-photodiode array detector-tandem mass spectrometry (HPLC-PAD-MS/MS) analysis showed the main components in the extract were 6-hydroxyluteolin-7-O-glucoside and rosmarinic acid, followed by luteolin-O-glucoside. After digestion process, the amount of total phenolic compounds (TPC) only decreased slightly, although a remarkable reduction in RA (near 50%) was detected. Bioavailable fraction contained 7.37 ± 1.39 mg/L digested extract of RA with small quantities of lithospermic acid and diosmin and presented an important antioxidant activity (0.89 ± 0.09 mmol Trolox/L digested extract). Besides, this bioavailable fraction produced a significant inhibition in TNF-α, IL-1β, and IL-6 secretion, using a human THP-1 macrophages model. Therefore, RA content in the basolateral compartment could play an important role in the antioxidant and anti-inflammatory activities found
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Google Scholar:Villalva Abarca, Marisol
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Jaime de Pablo, Laura
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Aguado, Estela
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Nieto, Juan Antonio
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Reglero Rada, Guillermo J.
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Santoyo Díez, Susana
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