DNA polymerases for whole genome amplification: considerations and future directions
Entity
UAM. Departamento de BioquímicaPublisher
MDPIDate
2023-05-26Citation
10.3390/ijms24119331
International Journal of Molecular Sciences 24.11 (2023): 9331
ISSN
1422-0067 (online); 1661-6596 (print)DOI
10.3390/ijms24119331Funded by
This research was funded by MCIN/AEI/10.13039/501100011033 and ERDF A way of making Europe, grant PID2021-123403NB-I00 to M.R.-RProject
Gobierno de España. PID2021-123403NB-I00Editor's Version
https://doi.org/10.3390/ijms24119331Subjects
Bst DNA polymerase; DNA polymerase; fidelity; multiple displacement amplification; PCR; processivity; translesion synthesis; whole genome amplification; Φ29 DNA polymerase; MedicinaRights
© 2023 by the authorsAbstract
In the same way that specialized DNA polymerases (DNAPs) replicate cellular and viral genomes, only a handful of dedicated proteins from various natural origins as well as engineered versions are appropriate for competent exponential amplification of whole genomes and metagenomes (WGA). Different applications have led to the development of diverse protocols, based on various DNAPs. Isothermal WGA is currently widely used due to the high performance of Φ29 DNA polymerase, but PCR-based methods are also available and can provide competent amplification of certain samples. Replication fidelity and processivity must be considered when selecting a suitable enzyme for WGA. However, other properties, such as thermostability, capacity to couple replication, and double helix unwinding, or the ability to maintain DNA replication opposite to damaged bases, are also very relevant for some applications. In this review, we provide an overview of the different properties of DNAPs widely used in WGA and discuss their limitations and future research directions
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Google Scholar:Ordóñez, Carlos D.
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Redrejo Rodríguez, Modesto
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