Sulphur and biothiol metabolism determine toxicity responses and fate of mercury in Arabidopsis
Entity
UAM. Departamento de BiologíaPublisher
SpringerDate
2021Citation
10.1016/j.envexpbot.2020.104302
Environmental and Experimental Botany 182 (2021): 104302
ISSN
0098-8472DOI
10.1016/j.envexpbot.2020.104302Funded by
Work supported by the Spanish State Research Agency (AEI) co-financed with the European Regional Development Fund (FEDER) (projects AGL2014-53771-R and AGL2017-87591-R to LEH and AGL2016-75226-R to AAF and JA; AEI/FEDER, UE). We thank Dr. M. Isabel Orús (Dept. Biology UAM, Madrid, Spain) for help in using the Scholander pressure chamber for obtaining xylem samplesProject
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014- 53771-R; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-87591-R; Gobierno de España. AGL2016-75226-REditor's Version
https://doi.org/10.1016/j.envexpbot.2020.104302Subjects
Plant mercury stress; Plant S-assimilation; Biothiol dynamics; Xylem Hg-PC complexes; Biología y Biomedicina / BiologíaRights
@2021 Springer Science-Business Media, LLC; part of Springer NatureEsta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Abstract
Mercury (Hg) is one of the most hazardous pollutants released by humans and is of global environmental concern. Mercury causes oxidative stress and strong cellular damages in plants, which can be attenuated by the biosynthesis of thiol-rich peptides (biothiols), including glutathione (GSH) and phytochelatins (PCs). We analysed Hg tolerance and speciation in five Arabidopsis thaliana genotypes, the wild-type Col-0, three knockdown γ-glutamylcysteine synthetase (γECS) mutants and a knockout PC synthase (PCS) mutant. Mercury-PC complexes were detected in roots by HPLC-ESI-TOFMS, with its abundance being limited in γECS mutants. Analysis of Hg-biothiol complexes in the xylem sap revealed that HgPC2 occurs in wild-type Col-0 Arabidopsis, suggesting that Hg could be translocated associated with thiol-rich metabolites. Twenty genes involved in sulphur assimilation, GSH and PCs synthesis were differentially expressed in roots and shoots, implying a complex regulation, possibly involving post-translational mechanisms independent of GSH cellular levels. In summary, the present study describes the importance of biothiol metabolism and adequate GSH levels in Hg tolerance and identifies for the first time Hg-PC complexes in the xylem sap. This finding supports the notion that Hg-biothiol complexes could contribute to Hg mobilisation within plants
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Google Scholar:Sobrino Plata, Juan
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Barón Sola, Ángel
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Ortega Villasante, Cristina
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Ortega Campayo, Víctor
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González Berrocal, César
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Conesa Quintana, Carlos
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Carrasco Gil, Sandra
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Muñoz Pinilla, María
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Abadía, Javier
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Álvarez Fernández, Ana
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Hernández, Luis E.
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